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Image Search Results
Journal: bioRxiv
Article Title: Expression and purification of the mitochondrial transmembrane protein FAM210A in Escherichia coli
doi: 10.1101/2023.05.27.542570
Figure Lengend Snippet: (A) Purification of full length FAM210A fusion protein from BL21(DE3) cells using a 0.25 mM IPTG induction for 3.5 hr at 37°C. Lane 1: Protein marker ladder. Lane 2: Uninduced cells. Lane 3: Induced cells. Lane 4: Cell debris pellet. Lane 5: Cell lysate flowthrough. Lane 6: Cell lysate supernatant. Lane 7: Cell lysate wash. Lane 8: Elution 1. Lane 9: Elution 2. Lane 10: Elution 3. Lane 11: Solubilized membrane flowthrough. Lane 12: Solubilized membrane wash. Lane 13: Membrane elution 1. Lane 14: Membrane elution 2. (B) Purification of dMTS FAM210A fusion protein using BL21(DE3) cells (100 mL) with 0.25 mM IPTG induction for 3.5 hr at 37°C. * is placed at the solubilized FAM210A fusion protein that was extracted from bacterial membranes. Lane 1: Protein marker ladder. Lane 2: Uninduced cells. Lane 3: Induced cells. Lane 4: Cell debris pellet. Lane 5: Cell lysate flowthrough. Lane 6: Cell lysate supernatant. Lane 7: Cell lysate wash. Lane 8: Elution 1. Lane 9: Elution 2. Lane 10: Elution 3. Lane 11: Solubilized membrane flowthrough. Lane 12: Solubilized membrane wash. Lane 13: Membrane Elution 1. Lane 14: Membrane elution 2.
Article Snippet: The solution of bound resin was transferred to a
Techniques: Purification, Marker, Membrane
Journal: bioRxiv
Article Title: Expression and purification of the mitochondrial transmembrane protein FAM210A in Escherichia coli
doi: 10.1101/2023.05.27.542570
Figure Lengend Snippet: Size exclusion chromatography purification of FAM210A-dMTS followed by Western blot analysis of fractions 1-6.
Article Snippet: The solution of bound resin was transferred to a
Techniques: Size-exclusion Chromatography, Purification, Western Blot